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to pediveligers (< 300 µm). If the veliger population size is not large enough to achieve desired densities, at least 30 veligers will be tested per replicate. Four exposure doses of EarthTec QZ will be investigated and a control will be included for each duration, with a maximum of three exposure durations. At least three replicates per exposure dose and duration will be completed and will be tested at a maximum of two temperatures. Once the exposure duration for each test condition is complete, the proportion of live and dead quagga mussels will be analyzed with fast green stain. In addition to the veliger assessment, each test beaker will be evaluated for copper (total and dissolved), dissolved oxygen, conductivity, pH, and temperature at the start of the experiment and at the end of the exposure time. Raw surface water samples will be monitored for total organic carbon (TOC), copper (total and dissolved), dissolved oxygen, conductivity, pH, and temperature. Task 2 Toxicity Assessment An assessment of the toxicity of EarthTec QZ for aquatic species — other than quagga veligers — present in the surface water will be completed in parallel with the dose -response veliger testing (Task 1). Trussell will consult with aquatic microbiology experts, CDFW staff, review available literature, and examine regulations to evaluate the water quality impacts of EarthTec QZ. In conjunction with Task 1 testing, surface water samples will be collected and dosed with EarthTec QZ to assess water quality impacts and toxicity. Slides will be prepared to communicate the proposed test plan. The toxicity testing will include up to three replicates with two doses of EarthTec QZ and assessment of toxicity for two aquatic indicator species. Task 3 Proiect Management The projectteam will work closely with OCWD and funding partners to coordinate testing events, provide updates of Study results, and communicate progress on Study activities. Trussell will attend up to eight meetings with project partners and prepare slides to communicate results and progress. Project staff will provide a presentation of the sudy findings at a symposium being held by MWD. 20C-32