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dose -response tests will be completed in field lab space close to the collection sites, with water <br />and veliger samples collected on the same day. <br />Veliger samples will first be assessed for density of live (physically moving or tissue movement of <br />veliger), dead (cracked shells or degraded tissue), or empty shell (with no tissue and only shell <br />remaining). If veliger concentrate contains >1% dead to live veliger density, the plankton <br />collection will not be used. The veliger concentrate will be divided into testing beakers to achieve <br />approximately 100 to 200 live veligers per test replicate, ranging in size from D-shaped (>63 µm) <br />to pediveligers (< 300 µm). If the veliger population size is not large enough to achieve desired <br />densities, at least 30 veligers will be tested per replicate. <br />Four exposure doses of EarthTec QZ will be investigated and a control will be included for each <br />duration, with a maximum of three exposure durations. At least three replicates per exposure <br />dose and duration will be completed and will be tested at a maximum of two temperatures. Once <br />the exposure duration for each test condition is complete, the proportion of live and dead quagga <br />mussels will be analyzed with fast green stain. In addition to the veliger assessment, each test <br />beaker will be evaluated for copper (total and dissolved), dissolved oxygen, conductivity, pH, and <br />temperature at the start of the experiment and at the end of the exposure time. Raw surface <br />water samples will be monitored for total organic carbon (TOC), copper (total and dissolved), <br />dissolved oxygen, conductivity, pH, and temperature. <br />Task 2 Toxicity Assessment <br />An assessment of the toxicity of EarthTec QZ for aquatic species - other than quagga veligers - <br />present in the surface water will be completed in parallel with the dose -response veliger testing <br />(Task 1). Trussell will consult with aquatic microbiology experts, CDFW staff, review available <br />literature, and examine regulations to evaluate the water quality impacts of EarthTec QZ. In <br />conjunction with Task 1 testing, surface water samples will be collected and dosed with EarthTec <br />QZ to assess water quality impacts and toxicity. Slides will be prepared to communicate the <br />proposed test plan. The toxicity testing will include up to three replicates with two doses of <br />EarthTec QZ and assessment of toxicity for two aquatic indicator species. <br />Task 3 Project Management <br />The projectteam will work closely with OCWD and funding partners to coordinate testing events, <br />provide updates of Study results, and communicate progress on Study activities. Trussell will <br />attend up to eight meetings with project partners and prepare slides to communicate results and <br />progress. <br />PR.os'�"I s'f/xFr- Wru- PRoJto�. � tz4z-c'S�n1T�'7i.x.7 � Trt� sway <br />Fr1a��S kt n SyM905.; 1A R.n J- 1yeLA2 0 <br />City of Santa Ana 28 Agreement No. 189280 <br />l7EtitJCW--Alt,t-vzS 0)SCc�l ' <br />