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In addition to examining the effectiveness of EarthTec QZ on quagga veligers, the Study includes <br />an assessment of the toxicity of EarthTec QZ for aquatic species — other than quagga veligers. <br />This work will be conducted in parallel with the dose -response veliger testing. Trussell will <br />consult with aquatic microbiology experts, CDFW staff, review available literature, and examine <br />regulations to evaluate the water quality impacts of EarthTec QZ. <br />Up to eight meetings are budgeted to ensure there is maximum collaboration with all Study <br />stakeholders at all stages of the Study. <br />Participating Entities and Supporting Entities <br />Participating entities include: <br />• Orange County Water District <br />• Water Replenishment District of Southern California <br />• United Water Conservation District <br />• Main San Gabriel Watermoster <br />• Central Basin Municipal Water District <br />• California Department of Fish and Wildlife (CDFW) <br />• California Department of Water Resources (DWR) <br />All the listed entities above, except for CDFW and DWR, are contributing financially to this Study. <br />CDFW and DWR are contributing staff time and providing technical assistance to the Study. <br />4.0 Description of Tasks <br />Task 1 Evaluation of EarthTec QZ Veliger Treatment <br />Bench scale testing will be completed at five surface water locations to establish quagga mussel <br />veliger dose -response curves with exposure to EarthTec QZ and determine the lowest <br />concentration that could be used to kill quagga veligers. <br />Testing is proposed at: <br />• Lake Matthews, <br />• OCWD's OC-28 connection in Anaheim, <br />• Lake Piru, and; <br />• MWD's CenB-28 connection <br />• One additional site to be identified in cooperation with Study partners. <br />Each site will be assessed for any available documentation of seasonal changes in veliger <br />populations, and sample collection and testing will be scheduled to target periods of high veliger <br />populations. Samples of raw surface water and quagga mussel veligers will be collected from <br />each of the surface water sites with plankton tow nets. To minimize veliger die -off, all veliger <br />City of Santa Ana 27 Agreement No. 189280 <br />